This was originally supposed to be isolated in 1975 from a frog Rana ridibunda (Kozuch et al., Acta virol. 22: 78, 1978), isolated and passaged on suckling mice and stored freeze-dried. This Sindbis virus[183-183-R2] is freeze dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group:
Infectivity tested and quantified
Infectivity was proven by plaque titration. Determined virus titer was 1,4x 10^6 PFU/ml.
Production cell line:
Passaged 7 times on suckling mice in 1980 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells and freeze dried on 17th october 2016.
Information about the collection of the virus
Biological material origin:
Collection date: BEFORE
Tuesday, 1 January, 1980
Country of collection: