TBEV-114


EVAg

Produced by
: BMC-SAS
Shipping From
: Bratislava - SK

Product Description

Ref-SKU:
008v-EVA1491
TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis virus[114] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group: 
Isolate: 

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Partial sequence
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 ml (2 vials of 0,5ml)

In stock

2 000,00 €
(Cost per access for Academics)

catalogue

biobank

Portal

EVAg

purchase

buy

order

price

cost

shipping

shipment

experiment

research

laboratory

viral

virus

GMO: 
No
Virus host type: 
Virus Cultivability: 
Infectivity Test: 
Infectivity was proven by plaque titration. Infectious titre was determined to be 2x 10^7 PFU/ml.
Viral titer: 
20000000 PFU/ml
Production cell line: 
Passage: 
Passaged 9 times on suckling mice on 29th May 1991 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells on 23rd August 2013.
Shipping conditions: 
Identification technique: 
RT-PCR followed by sequencing of the amplified product.
IATA Classification: 
Technical recommendation: 
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,1 can be used for the infection and the infectious medium can be harvested 96 hours after infection.

Information about the collection of the virus

Biological material origin: 
Natural origin
Collection date: BEFORE 
Tuesday, 1 January, 1980
Country of collection: 
Suspected epidemiological origin : 
Isolation host: