TBEV-Clg223


EVAg

Produced by
: BMC-SAS
Shipping From
: Bratislava - SK

Product Description

Ref-SKU:
008v-EVA1493
TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis[Clg223] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group: 
Isolate: 

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Partial sequence
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 ml (2 vials of 0,5ml)

In stock

2 000,00 €
(Cost per access for Academics)

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GMO: 
No
Virus host type: 
Virus Cultivability: 
Infectivity Test: 
Infectivity was proven by plaque titration. Infectious titre was determined to be 1,3x 10^7 PFU/ml.
Viral titer: 
13000000 PFU/ml
Production cell line: 
Passage: 
Passaged 5 times on suckling mice on 4th September 1992 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells on 23rd August 2013.
Identification technique: 
RT-PCR followed by sequencing of the amplified product.
Technical recommendation: 
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 1 can be used for the infection and the infectious medium can be harvested 96 hours after infection.
Shipping conditions: 
IATA Classification: 

Information about the collection of the virus

Biological material origin: 
Natural origin
Collection date: BEFORE 
Tuesday, 1 January, 1980
Country of collection: