Human anti-HCoV-OC43 Spike protein (S2) IgG1, monoclonal antibody (clone BCP002)
Produced by: ERASMUS-MC
Shipping From: Rotterdam - NL
Human monoclonal recombinant IgG1 targeting the S2 domain of HCoV-OC43 Spike domain, as determined by ELISA.
Product Risk Group:
Information on the related virus
Antibody purified by affinity:
Human HCoV-OC43 Spike specific B-cells were identified through culturing at limiting dilution. Heavy and Light variable sequences were amplified. After amplification the variable regions were cloned into Heavy and Light pFUSEss expression plasmids. The antibody was expressed by co-transfecting 293T cells with both plasmids. Secreted antibody was subsequently affinity purified and concentrated.
Antibody characterization method(s):
Information on characterization:
The antibody showed reactivity towards HCoV-OC43 S-trimer (Sino Biological; 40607-V08B) and S2 domain (Sino Biological; 40607-V08B1). Antibody showed high affinity for S2 as determined by ELISA.
Virus host type:
This antibody is specific for the S2 domain of spike protein of HCoV-OC43 virus.