2019-nCoV E gene stabilized RNA as positive control: shipping at Room Temperature
detection-kit (RUO)

2019-nCoV E gene stabilized RNA as positive control: shipping at Room Temperature

EVAg
Produced by
: AMU
Shipping From
: Marseille - FR

Product Description

Ref-SKU:
001K-03884
Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the E gene, for 100 Rxns (Target designed by Charité Universitätsmedizin Berlin ; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)
Product Risk Group: 
No RG

Information on the related virus

ICTV Taxonomy: 
Riboviria / Orthornavirae / Pisuviricota / Pisoniviricetes / Nidovirales / Cornidovirineae / Coronaviridae / Orthocoronavirinae / Betacoronavirus / Sarbecovirus / Severe acute respiratory syndrome-related coronavirus
Virus name: 
SARS-CoV-2


Unit definition: 
1 vial

In stock

200,00 €
 (Cost per access for Academics)
Free access available

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Can it be used to produce GMO: 
No
Shipping conditions: 
Room Temperature
IATA Classification: 
NON DANGEROUS GOODS
Storage conditions: 
Freeze Dried (-20C)
Targeted region: 
E gene of the SARS-CoV-2
Technical recommendation: 
Extraction is mandatory before use in real time RT-PCR
Specificity documented: 
Yes
Specificity: 
SOP
SOP File: 
PDF icon ArRNASOP final.pdf
Previous Name or Taxonomy: 
2019-nCoV

Comments (10)

  • Adamu Mekonnen's picture

    For the detection of the NCOV 2019

    Feb 09, 2020
  • Adamu Mekonnen's picture

    Thanks

    Feb 09, 2020
  • C.Baronti's picture

    Dear Adamu,

    The encapsidated RNA is a positive control in the E gene of the 2019-nCoV which could be used for control in real time RT-PCR. We provide also Primers and Probe for the detection of 2019-nCoV (001K-03885)

    sincerely,

    Feb 10, 2020
  • nicolas.hougardy@vivalia.be's picture

    I am looking for a positive control of the RdRp gene. Do you have some avaliable?
    Sincerely

    Feb 25, 2020
  • C.Baronti's picture

    Dear Nicolas,

    Thank you for your interest in our products.

    Positive control of the RdRp gene will be soon available on the EVAg website (about 10J).  I will keep you informed.

    Sincerely,

    Feb 25, 2020
  • C.Baronti's picture

    Dear Nicolas,

    Positive control and primers/probe of the RdRp gene are now available on the site. (ref products: 001K-03906, 001K-03905)

    sincerely,

    Mar 19, 2020
  • nadegepelte's picture

    Hello,
    What is the target region of this positive control? Is it the entire E gene? a portion? Any 5' or 3' extra sequence?
    Thank you
    Nadege

    Mar 19, 2020
  • C.Baronti's picture

    The target of the positive control is a portion of the E gene as describe in the paper  https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf

    sincerely

    Mar 19, 2020
  • nadegepelte's picture

    OK thank you.

    Mar 19, 2020
  • elisa.saccon's picture

    Hello, I read in the SOP that "A vial contains 1.5x10^7 copies suitable for ~100 reactions". Is this before rehydration? How can I quantify how many copies I have after extraction?
    Thank you for your help
    Elisa

    Oct 12, 2020