Training in reverse genetics method (ISA method), recently developed to reconstitute an infectious Flavivirus or Alphavirus under BSL-3 conditions.
The training comprises DNA fragments amplification by PCR; Transfection of the purified products into permissive cells; Viral culture; Molecular detection
Freeze-dried Japanese encephalitis virus, strain UVE/JEV/UNK/TW/RP9-190, Taiwan, complete ORF sequence. It was produced by ISA method from Infectious clone RP-9 (PMID:8873412)
Freeze-dried Chikungunya virus, strain UVE/CHIKV/2009/FR/15.3 WT. This strain was obtained from modified infectious clone (LR2006_OPY1; La Reunion island 2006), transfected and cultivated on Vero cells
Freeze-dried SARS-CoV-2 virus, strain SARS-CoV-2/2021/FR/0610 (Lineage B 1.617.2, Delta) France 2021, complete genome sequence.
Presence of spike mutation A222V and absence of spike mutation Y145H.
Freeze-dried Yellow fever virus, strain UVE/YFV/1927/GN/Asibi, complete ORF sequenced . It was produced by ISA method from "AY640589" genbank sequence.