EVAg

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect castor bean tick nymphs (Ixodes ricinus). The nymphs will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 nymphs will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 nymphs and provided to the end-user.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect adult castor bean ticks (Ixodes ricinus). The ticks will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 ticks will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 ticks and provided to the end-user.

Bundle of freeze-dried tick-borne encephalitis virus (TBEV) isolates representing the diversity of the virus in Central Europe and covering various isolation sources (tick, rodent, milk).

Chikungunya virus cDNA

Tick-borne encephalitis virus strain Hypr cDNA

Tick-borne encephalitis virus strain Hypr RNA

HTNV 78-118 cDNA to be used as a positive control for PCR. Total RNA prepared from cell culture supernatant and transcribed using random hexamers.

Hantaan virus RNA

West Nile virus cDNA.

Langat virus cDNA

West Nile E13 virus cDNA

Tick-borne encephalitis virus, strain RSSE cDNA

Negishi virus cDNA

Louping ill virus cDNA

Japanese encephalitis virus cDNA.

Eastern equine encephalitis virus 2090 cDNA

Sindbis virus (strain 183-SK2) cDNA

Sindbis virus (strain 183-R2) cDNA

Sindbis virus (strain 183) cDNA

Semliki forest virus cDNA.

Tribec virus cDNA

Tula virus [Moravia/5302Ma/94] cDNA to be used as a positive control for PCR. Total RNA prepared from cell culture supernatant and transcribed using random hexamers.

Tick-borne encephalitis virus, isolate 285 cDNA

Tick-borne encephalitis virus, isolate 285 RNA.

DOBV Slovakia/Aa cDNA to be used as a positive control for PCR. Total RNA prepared from cell culture supernatant and transcribed using random hexamers.