EVAg

The detection of viral pathogens infecting a given plant sample, obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology or for the certification of seed and planting material. The service offers the individual analysis of three plant samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

Development and production of Diagnostic systems with positive control for arboviruses (RUO)

Production on request of stabilisated RNA positif control, as known as Armored RNA for RT-PCR. This service includes: - Design of the positif control sequence based on the sequence provided by the costumer - Production and lyophilisation of Armored RNA according the quantity needed - LOD/quantification - Shipment of the Armored RNA at room temperature

Specific tests for certain regulated viruses are required for the certification of seed and planting material. However, the main limitation of this approach is that only these viruses are detected. Latent and/or unknown viruses, which can also have a significant impact on yield and product quality, remain unidentified. Especially in vegetative propagation material, this inevitably leads to the accumulation of such pathogens. High-throughput sequencing and bioinformatics analysis offers a global analysis of the phytosanitary status of plants.

Generation of recombinant chimeric monoclonal antibodies: murine monoclonal antibodies (hybridoma cells or total RNA have to be provided by the end-user) will be sequenced, cloned, recombinantly expressed in Expi293 cells, and purified via protein G. Available backbones for heavy and light chain constant regions are: mouse IgG1, Ig kappa; mouse IgG2a, Ig kappa; rabbit IgG, Ig kappa; human IgG1, Ig kappa; porcine IgG, Ig kappa; and bovine IgG, Ig kappa. Other backbones could possibly also generated uppon request.

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in whole blood and serum samples. Provided with Lyophilized ready-to-use primers and probe for real time RT-PCR in a region of the MS2 phage. Positive control packaged for 100 Rxns; P&P packaged for 96 rxns in the form of 2x48 rxns Disclaimer: This product does not contain the polymerase. For RUO (Research Use Only).

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in nasopharyngeal, salivary, urine and stool samples. Provided with Lyophilized ready-to-use primers and probe for real time RT-PCR in a region of the MS2 phage. Positive control packaged for 100 Rxns; P&P packaged for 96 rxns in the form of 2x48 rxns Disclaimer: This product does not contain the polymerase. For RUO (Research Use Only).

Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 2x48 rxns) for real time RT-PCR in a region of the MS2 phage for internal control. Disclaimer: This product does not contain the polymerase For RUO (research use only)

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in whole blood and serum samples. Extraction is mandatory before use in real time RT-PCR. Packaged for 100 Rxns, for RUO (Research Use Only). SOPs in the attached pdf.

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in nasopharyngeal, salivary, urine and stool samples. Extraction is mandatory before use in real time RT-PCR. Packaged for 100 Rxns, for RUO (Research Use Only). SOPs in the attached pdf.

In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens.

High-throughput sequencing and bioinformatics analysis of samples for a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens. Can be used for the discovery of novel or rare virus variants, the characterization of the genetic variation and the population composition.

Access to AMU/AFMB infrastructure for learning/training on techniques related to viral recombinant proteins

RG2 virus production (available on the EVAg catalogue) in single batch upon request containing 30 freeze-dried vials. The specific detection system (5 vials) by real-time PCR will be provided in the same package.

Institut Pasteur Paris - Access to BSL 3 containment facility. The access will comprise a detailed theoretical and subject to availability practical application of the biosafety and biosecurity management at Institut Pasteur. Among others, establishment and validation of suitable decontamination methods for waist, rooms and material, as well as documentation and information management will be addressed.

Kidney cells (epithelial)/ fibroblast–like, origin: Chlorocebus sabaeus (African green monkey); CRISPR/Cas9-engineered IFN-Lambda deficient phenotype

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

Institut Pasteur Paris - Access to BSL 3 containment facility. The access will comprise a detailed theoretical and practical application of different methods to grow lyssaviruses: strain isolation from clinical sample, virus adaptation to cell culture and Virus titration Access to BSL 3 containment facility. The access will comprise a detailed theoretical and practical application of different methods to grow lyssaviruses: strain isolation from clinical sample, virus adaptation to cell culture and Virus titration

Overall presentation of sequening workflow using S5 device. From extraction to bio-informatics data treatment. Extraction, amplification purification and quantification of viral nucleic acid. Library building- chef manipulations- S5 initialisation, torrent programmation- Bio-informatics treatment of the data using CLC genomics software

Access to large animal high containment facilities BSL 3 and BSL 4 (vet). The access will comprise a detailed theoretical and subject to availability practical application of the biosafety and biosecurity management of the Friedrich-Loeffler-Institut. Among others, handling of large animals, necropsy including tissue sampling, establishment and validation of suitable decontamination methods for rooms and HEPA filters in animal high containment facilities, as well as documentation and information management will be addressed.

In this 3-day course the basic principles of routine virus typing will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses. Typing of enteroviruses will be used as an example, but the general procedures are applicable to typing of other viruses also. After this course participants should have sufficient knowledge to introduce routine typing of viruses in their own laboratory.

Access to our corresponding bio-bank laboratories. Detailed presentation of our two different established management systems of the accredited virus and cell culture collection of the Friedrich-Loeffler-Institute. This will include the theoretical demonstration and practical performance of individually selected standard operating procedures together with the end-user. Thereby, the focus will be on our established quality management procedures, but also main aspects of biosafety and biosecurity will be addressed.

General description of the technique and required skills and equipment. Access to BSL2 laboratories. Reminder of biosafety and biosecurity procedures. Preparation of Vero cells in an adapted format. Preparation of sera and titrated virus. Read-out of the results and interpretation.

Access to rooms in animal facilities to perform animal trials up to conditions of risk group 2 (public health or animal health). In our animal facilities we could accommodate e.g. fish, sheep, goats, pigs, cattle or poultry.