EVAg

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect castor bean tick nymphs (Ixodes ricinus). The nymphs will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 nymphs will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 nymphs and provided to the end-user.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect adult castor bean ticks (Ixodes ricinus). The ticks will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 ticks will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 ticks and provided to the end-user.

Bundle of freeze-dried tick-borne encephalitis virus (TBEV) isolates representing the diversity of the virus in Central Europe and covering various isolation sources (tick, rodent, milk).

Bundle of freeze-dried SARS-CoV-2 isolates representing the course of the pandemic in Central Europe including the original virus, early variant B.1.258, apha, beta, delta VOCs, and several omicron variants. The composition of the bundle can be modified upon request.

Virus production of viruses available on the EVAg catalogue from BMC-SAS in a single batch upon request containing 30 cryotubes containing 0.5mL.