Production on request of stabilisated RNA positif control, as known as Armored RNA for RT-PCR. This service includes:
- Design of the positif control sequence based on the sequence provided by the costumer
- Production and lyophilisation of Armored RNA according the quantity needed
- LOD/quantification
- Shipment of the Armored RNA at room temperature
The lyophilized, non infectious, encapsidated RNA (Armored RNA) contains the sequence of the 3'UTR and the pro-Capside region from West Nile virus and can be used as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. Packaged for 100 Rxns, for RUO (Research Use Only). SOPs in the attached pdf.
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 180 rxns in the form of 10x18 rxns) for real time RT-PCR of Multiplex Dengue Serotyping (Taqman)
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
genomic RNA from culture supernatant of West Nile virus strain UVE/WNV/1999/US/NY 385-99
50µL provided in AVE buffer (without RNA carrier), contains 2.15 x 10^8 RNA cp/µl
genomic RNA from culture supernatant of West Nile virus strain UVE/WNV/1940/UG/UG 956 D117
50µL provided in AVE buffer (without RNA carrier), contains 1.01 x 10^8 RNA cp/µl
Freeze-dried Japanese encephalitis virus, strain UVE/JEV/UNK/TW/RP9-190, Taiwan, complete ORF sequence. It was produced by ISA method from Infectious clone RP-9 (PMID:8873412)
Freeze-dried Chikungunya virus, strain UVE/CHIKV/2009/FR/15.3 WT. This strain was obtained from modified infectious clone (LR2006_OPY1; La Reunion island 2006), transfected and cultivated on Vero cells
