ISA Reverse Genetic System for chimeric virus CH-17D / ZIKV


Product Description

Ref-SKU:
001N-03399
Infectious Subgenomic Amplicons Reverse genetic system for chimeric virus CH-17D / ZIKV. It consists of three non infectious overlapping DNA fragments (cloned into pUC57 vector) . These products enable to obtain infectious RNA virus after amplification by PCR, and transfection into susceptible cells. The chimeric virus systems were constructed using the yellow fever 17-D vaccine strain as a genetic backbone and by replacing prM/E from this vaccine strain with those of the Asian ZIKV PF epidemic strain. As the structural proteins are all located in the first fragment, this one is the chimeric fragment, the two others are from YFV-17D vaccine system.
Product Risk Group: 

Information on the related virus

Strain: 
CH-17D / ZIKV


Unit definition: 
3 overlapping plasmids

In stock

900,00 €
(Cost per access for Academics)

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Cloned Product: Yes
Can it be used to produce GMO: 
Yes
Biosafety restrictions: 
The simultaneous transfection of ISA overlapping fragments covering the entire genome of a virus must be performed in the biosafety environnement required for the culture of the corresponding virus
Region encompassed in this Product: 
full genome
Plasmid: 
Plasmid selection: 
TAG: 
Sequencing: 
Fully sequenced
Sequence controlled: 
Yes
Mutations: 
No mutation compared to the reference sequence
Storage conditions: 
Nuclease-Free Water -20C
Shipping conditions: 
IATA Classification: