Infectious cell culture supernatant containing HIV-1 subtype CRF12_BF strain HIV-1_DABP_CFR12_BF_INMI. The strain was obtained after co-culture of patient plasma with CD8-depleted PBMC from healthy blood donors. This strain is preserved under Viral Storage Medium -80C and is Mycoplasma free. To confirm its identity the virus has been partially sequenced.
Product Risk Group:
Subtype CFR12_BF. Mutations: pr-K20R, pr-M36I, pr-I93L, rt-E138A
Viral Storage Medium -80C
Infectivity tested and quantified
Virus production after co-culture of patient plasma with CD8-depleted donor PBMC. Virus production in the supernatant was tested with HIV-1 viral RNA qPCR
Production cell line:
HIV-1 qPCR on culture supernatant and sequencing