Infectious cell culture supernatant containing HIV-1 subtype A1 strain HIV-1_RoLu_A1_INMI. The strain was obtained after co-culture of patient plasma with CD8-depleted PBMC from healthy blood donors. This strain strain is preserved under Viral Storage Medium -80C. To confirm its identity the virus has been partially sequenced.
Product Risk Group:
Subtype A1. Mutations: pr-K20R, pr-M36I, pr-L89M, rt-V179T.
Viral Storage Medium -80C
Infectivity tested and quantified
Virus production after co-culture of patient plasma with CD8-depleted donor PBMC. Virus production in the supernatant was tested with p24 ELISA
Production cell line:
PCR and sequencing