Human anti-SARS-CoV-2 Spike protein (S1) IgG1, monoclonal antibody (clone BCP005)


EVAg

Produced by
: ERASMUS-MC
Shipping From
: Rotterdam - NL

Product Description

Ref-SKU:
010A-05021
Human monoclonal recombinant IgG1 targeting the S1 domain of SARS-CoV-2 Spike as determined by ELISA.
Product Risk Group: 

Information on the related virus

Virus name: 


Unit definition: 
1 vial, ~250 µL, 68,6 µg/mL, 17,2 µg

Upon request

500,00 €
(Cost per access for Academics)

catalogue

biobank

Portal

EVAg

purchase

buy

order

price

cost

shipping

shipment

experiment

research

laboratory

viral

virus

Biosafety restrictions: 
none
Product type: 
Antibody
Targeted antigen: 
Virus
Antibody type: 
Monoclonal
Antibody purified by affinity: 
Yes
Production system: 
Human SARS-CoV-2 Spike specific single B-cells were FACS sorted. After amplification the variable regions were cloned into Heavy and Light pFUSEss expression plasmids. The antibody was expressed by co-transfecting 293T cells with both plasmids. Secreted antibody was subsequently affinity purified and concentrated.
Antibody characterization method(s): 
ELISA
Information on characterization: 
The antibody showed reactivity towards ancestral SARS-CoV-2 S-trimer (Sino Biological; 40589-V08H8) and S1 (Sino Biological; 40591-V08H3) but not to RBD (Sino Biological; 40592-V08H) as determined using ELISA. Antibody showed high affinity for S1 as determined by ELISA. Binding to ancestral SARS-CoV-2 antigens was verified on protein micro array (Aguilar-Bretones et al., JCI, 2021). Binding to the Delta and Omicron BA.5 variants was detected but not to Omicron BA.1. No cross-reactivity to hCoV-OC43, hCoV-NL63, hCoV-HKU1, hCoV-229E was detected.
Specificity documented: 
Yes
Virus host type: 
Antibody specificity: 
This antibody is specific for the S1 domain of spike protein of SARS-CoV-2 virus.
Previous Name or Taxonomy: 
Shipping conditions: 
IATA Classification: