Human anti-SARS-CoV-2 Spike protein (S2) IgG1, monoclonal antibody (clone BCP007)


EVAg

Produced by
: ERASMUS-MC
Shipping From
: Rotterdam - NL

Product Description

Ref-SKU:
010A-05022
Human monoclonal recombinant IgG1 targeting the S2 domain of SARS-CoV-2 Spike as determined by ELISA.
Product Risk Group: 

Information on the related virus

Virus name: 


Unit definition: 
1 vial, ~200 µL, 51,6 µg/mL, 10,3 µg

Upon request

500,00 €
(Cost per access for Academics)

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Biosafety restrictions: 
none
Product type: 
Antibody
Targeted antigen: 
Virus
Antibody type: 
Monoclonal
Antibody purified by affinity: 
Yes
Production system: 
Human SARS-CoV-2 Spike specific single B-cells were FACS sorted. After amplification the variable regions were cloned into Heavy and Light pFUSEss expression plasmids. The antibody was expressed by co-transfecting 293T cells with both plasmids. Secreted antibody was subsequently affinity purified and concentrated.
Antibody characterization method(s): 
ELISA
Information on characterization: 
The antibody showed reactivity towards the ancestral SARS-CoV-2 S-trimer(Sino Biological; 40589-V08H8) and S2 domain (Sino Biological; 40590-V08H1). Antibody showed high affinity for S2 as determined by ELISA. Binding to ancestral SARS-CoV-2 was also verified on protein array (Aguilar-Bretones et al., JCI, 2021). Binding to the Omicron BA.1 and BA.5 variants was detected, but not towards Delta. No cross-reactivity to hCoV-OC43, hCoV-NL63, hCoV-HKU1, hCoV-229E was detected. Antibody purity was assessed on SDS-PAGE and showed >95% purity.
Specificity documented: 
Yes
Virus host type: 
Antibody specificity: 
This antibody is specific for the S2 domain of spike protein of SARS-CoV-2 virus.
Previous Name or Taxonomy: 
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IATA Classification: