EVA

Expression Plasmid of TGEV Nsp9, Full gene (RNA binding protein), in fusion with a N-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp9, Full gene (RNA binding protein), in fusion with a C-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp8, Full gene (UNK), in fusion with a N-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp8, Full gene (UNK), in fusion with a C-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp7, Full gene (UNK), in fusion with a N-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp7, Full gene (UNK), in fusion with a C-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp5, Full gene (Protease), in fusion with a N-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp5, Full gene (protease), in fusion with a C-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp13, Full gene (Helicase), in fusion with a C-Terminal His tag coding sequence for expression in E. coli

Expression Plasmid of TGEV Nsp10, Full gene (UNK), in fusion with a N-Terminal His tag coding sequence for expression in E. coli

Through direct cloning and expression of the variable heavy chains (VHHs) of HCAbs from the bone marrow of MERS-CoV infected dromedaries, MERS-CoV specific VHHs or nanobodies were produced. VHH-83 binds with exceptionally high affinity to the receptor binding domain of the viral spike protein. In vitro, this VHH efficiently blocks virus entry at picomolar concentrations

Serum obtained from dromedary camels immunized with a MVA vaccine expressing the spike protein and challenged with MERS-CoV.

HCAb-83 is a MERS-CoV specific camel/human chimeric heavy chain only antibody (HCAb) - composed of the camel VHH-83 linked to a human Fc domain lacking the CH1 exon Through direct cloning and expression of the variable heavy chains (VHHs) of HCAbs from the bone marrow of MERS-CoV infected dromedaries, MERS-CoV specific VHHs or nanobodies were produced. VHH-83 binds with exceptionally high affinity to the receptor binding domain of the viral spike protein. In vitro, HCAb-83 efficiently blocks virus entry at picomolar concentrations

Panel consists of (cell-culture) RNA from different coronaviruses (HCoV-Nl63, HCoV-229E, HCoV-OC43, MERS-CoV and SARS-CoV) and is intended for use as control of specificity of molecular assays. This panel contains sufficient RNA for t least 5 experiments. "Disclaimer: Import permit may be needed in your country for SARS-CoV full virus RNA. If yes, please indicate so in your online enquiry". Product for Alphacoronavirus and Betacoronavirus. ------------------ NB: indicated price is only valid for non-profit laboratories. Commercial users will be contacted about pricing.

N-terminal domain of the MERS CoV nucleocapsid (1-164). Pure recombinant protein produced in E. coli. resuspended in HEPES buffer 10 mM pH 7.5, NaCl 300 mM.

N-terminal domain of the MERS CoV nucleocapsid (37-164, without the N-terminal intrinsically disordered region. Pure recombinant protein produced in E. coli. resuspended in HEPES buffer 10 mM pH 7.5, NaCl 300 mM

Expression plasmid for the N-terminal Domain of the MERS CoV Nucleocapsid coding sequence in fusion with a TRX/6-His tag encoding sequence at its 5'. For expression in E. coli

Expression plasmid for the Nterminal Domain of the MERS CoV Nucleocapsid coding sequence in fusion with a TRX/6-His tag encoding sequence at its 5'. For expression in E. coli

In vitro transcribed RNA for a specific SARS-coronavirus real time PCR.

Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Netherlands/ZuidHolland_10004/2020, D614G (S)). This strain is preserved in viral storage medium -80°C.

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the N (CUB) gene, for 100 Rxns (Target designed by Charité Universitätsmedizin Berlin). The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)

N-terminal domain of the SARS-CoV-2 nucleprotein (1-180). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.

C-terminal domain of the SARS-CoV-2 nucleprotein (247-418). Pure recombinant protein produced in E. coli and fused with a Avi tag and a Hexa-Hisitidine tag.

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

Lyophilized encapsidated RNA for SARS-CoV-2 (2019-nCoV) real time RT-PCR targeting region of the RdRp (IP2/IP4) gene, for 100 Rxns (Target designed by Institut Pasteur: https://www.who.int/docs/default-source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-pasteur-paris.pdf?sfvrsn=3662fcb6_2) The encapsidated RNA has been stabilized to serve as positive control for extraction and real time RT-PCR. Extraction is mandatory before use in real time RT-PCR. SOPs in the attached pdf. For RUO (Research Use Only)