Access to virus whole genome sequencing service at APHA


EVAg

Produced by
: DEFRA
Shipping From
: Addlestone - GB

Product Description

Ref-SKU:
003S-02038
Virus whole genome sequencing
Product Risk Group: 

Information on the related virus

ICTV Taxonomy: 
Virus name: 


Unit definition: 
Provision of consensus genome sequence for one virus sample. This is a guide price and a quote will be provided upon request. Economies of scale may be applicable when sequencing multiple samples at the same time.

Available within 3 months

3 500,00 €
(Cost per access for Academics)

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Can it produce GMO: 
No
Technical recommendation: 
We request that purified DNA/RNA is submitted for sequencing. This can either be from tissue culture or clinical material, but we will need assurances that the the material is not infectious. If RNA samples are submitted, please deplete genomic DNA and ribosomal RNA, as this will improve the proportion of viral sequence reads recovered (see Marston et al, 2013: https://dx.doi.org/10.1186/1471-2164-14-444 for our recommended extraction and depletion methods). Samples will be sequenced on the Illumina MiSeq platform and the consensus of the viral genome will be generated from the raw data using our in-house data processing pipeline. Full details of the process are available on request.

Comments (2)

  • Chiaram's picture

    We request whole genome sequencing of influenza virus genomic RNA
    We will provide APHA with a NON infectious RNA sample.

    Aug 01, 2019
  • L.Thorne's picture

    Dear Chiaram,

    Apologies, I completely missed your comment regarding NGS sequencing of influenza virus. This is soemthing we can do for you. If you are still interested please get back to me using my e-mail address leigh.thorne@apha.gov.uk .

    Apologies again,

    Leigh Thorne

    APHA

    Oct 14, 2019