EVAg

Lyophilized encapsidated RNA (ArRNA) contains a region of the MS2 phage and is used as positive control for extraction and real time RT-PCR. The ArRNA is especially developed to be spiked in nasopharyngeal, salivary, urine and stool samples. Extraction is mandatory before use in real time RT-PCR. Packaged for 100 Rxns, for RUO (Research Use Only). SOPs in the attached pdf.

In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens.

High-throughput sequencing and bioinformatics analysis of samples for a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens. Can be used for the discovery of novel or rare virus variants, the characterization of the genetic variation and the population composition.

Access to AMU/AFMB infrastructure for learning/training on techniques related to viral recombinant proteins

SARS-CoV-2 diagnostic training, including differential diagnostics of human Coronaviruses and other respiratory pathogens, incl. BSL-2 and BLS-3 conditions.

RG2 virus production (available on the EVAg catalogue) in single batch upon request containing 30 freeze-dried vials. The specific detection system (5 vials) by real-time PCR will be provided in the same package.

Institut Pasteur Paris - Access to BSL 3 containment facility. The access will comprise a detailed theoretical and subject to availability practical application of the biosafety and biosecurity management at Institut Pasteur. Among others, establishment and validation of suitable decontamination methods for waist, rooms and material, as well as documentation and information management will be addressed.

In this 3-day course the basic principles of the protein microarray will be addressed by a combination of lectures, demonstration and hands-on training. The protein microarray technique can be used for HA antibody profiling for group level assessment of exposures to influenza viruses in small sample volumes from humans and animals (birds, horses, pigs, bats, carnivores a.o.). The training encompasses wet lab training but also the analysis and interpretation of assay results. Special attention will be given to specific issues of Influenza and flavivirus cross reactivity.

Diagnostic training in zooonotic viruses under BSL-2/BSL-3 conditions at the Institute of Virology - Charité Universitätsmedizin Berlin, Germany.

Comparative arbovirus diagnostics training, including molecular and serologic techniques under BSL-2 and BSL-3 conditions

Kidney cells (epithelial)/ fibroblast–like, origin: Chlorocebus sabaeus (African green monkey); CRISPR/Cas9-engineered IFN-Lambda deficient phenotype

The generation of an antigen-specific murine monoclonal hybridoma cell line against a specific antigen provided by the end-user (purified Protein or inactivated purified virus) included: 1. the immunization of 2 BALB/c mice, 2. the fusion of the spleen cells with SP2/0 cells, 3. Screening and selection of suitable clones (~ 10 to 15) by using a suitable screening method depending of the planned later usage, 4.

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

In this 3-day course the basic principles of serological testing against ARBO viruses will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all laboratory techniques including ELISA, Immunofluorescence and virus neutralization. Attention will be given to serological testing in cerebrospinal fluid, including the calculation of an antibody index.

Access to the BSL-3 facility including equipment and personal protective equipment, theoretical and practical training; preparation and evaluation of the BSL-3 work; BSL-3 virus collection as predefined within the project of the end user; personal support for performing specifically designed experiments; expert supervision.

Institut Pasteur Paris - Access to BSL 3 containment facility. The access will comprise a detailed theoretical and practical application of different methods to grow lyssaviruses: strain isolation from clinical sample, virus adaptation to cell culture and Virus titration Access to BSL 3 containment facility. The access will comprise a detailed theoretical and practical application of different methods to grow lyssaviruses: strain isolation from clinical sample, virus adaptation to cell culture and Virus titration

Institut Pasteur Paris - General description of the RFFIT and required skills and equipment. Preparation of cells, sera and titrated virus. Read-out of the results and interpretation.

Institut Pasteur Paris - General description of fluorecent antibody tets (FAT), virus isolation, lateral flow device in the context of rabies diagnosis and surveillance. Practical training in these technique in BSL3.

Institut Pasteur Paris - In this 2 weeks course the basic principles of routine virus typing will be addressed by a combination of lectures, demonstration and hands-on training. Participants will perform all required laboratory and analysis procedures for routine typing of lyssaviruses: Special attention will be given to quality control and assay validation.

Overall presentation of sequening workflow using S5 device. From extraction to bio-informatics data treatment. Extraction, amplification purification and quantification of viral nucleic acid. Library building- chef manipulations- S5 initialisation, torrent programmation- Bio-informatics treatment of the data using CLC genomics software

Access to large animal high containment facilities BSL 3 and BSL 4 (vet). The access will comprise a detailed theoretical and subject to availability practical application of the biosafety and biosecurity management of the Friedrich-Loeffler-Institut. Among others, handling of large animals, necropsy including tissue sampling, establishment and validation of suitable decontamination methods for rooms and HEPA filters in animal high containment facilities, as well as documentation and information management will be addressed.

In this 3-day course the basic principles of routine virus typing will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses. Typing of enteroviruses will be used as an example, but the general procedures are applicable to typing of other viruses also. After this course participants should have sufficient knowledge to introduce routine typing of viruses in their own laboratory.

Access to our corresponding bio-bank laboratories. Detailed presentation of our two different established management systems of the accredited virus and cell culture collection of the Friedrich-Loeffler-Institute. This will include the theoretical demonstration and practical performance of individually selected standard operating procedures together with the end-user. Thereby, the focus will be on our established quality management procedures, but also main aspects of biosafety and biosecurity will be addressed.

General description of the technique and required skills and equipment. Access to BSL2 laboratories. Reminder of biosafety and biosecurity procedures. Preparation of Vero cells in an adapted format. Preparation of sera and titrated virus. Read-out of the results and interpretation.