Course: From sequencing to sequence analysis
This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses. Typing of enteroviruses will be used as an example, but the general procedures are applicable to typing of other viruses also. After this module participants should have sufficient knowledge to introduce routine typing of viruses in their own laboratory. Special attention will be given to quality control and assay validation. Topics included: - PCR workflow - Nucleic Acid isolation - Reverse transcription and PCR - Sequencing and sequence analysis - Basic phylogeny for virus typing - Assay validation and Quality control in molecular diagnostics The second module is a hands-on three-day course which focuses on the analysis of sequences. After completion of the second analytical module, participants will be able to i) understand the statistical and biological background of sequence analysis including BLAST and phylogenetic approaches including neighbour-joining, maximum likelihood and BEAST analysis, and ii) to independently perform and evaluate complex sequence analysis. Participants are invited to work under supervision on their own sequences.
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