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Produced by
: DSMZ Shipping From
: Braunschweig - DE
Product Description
Ref-SKU:
027S-05487 In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens. This service package offers the individual analysis of ten plant samples by HTS to ensure a reliable detection of the potentially involved viruses in an outbreak situation.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Virus host type:
Can it produce GMO:
No
Comments (4)
Citrus is one of the most important fruit crops in Jordan. The most important citrus species grown in Jordan are sweet orange, mandarins and mandarin-like citrus, lemon and grapefruit. Approximately 98% of all these species are planted on sour orange root-stock, and most of the citrus cultivars are grown as grafted plants. As a consequence, virus pathogens transmitted by grafting as well as by insect vectors can cause diseases which finally affect fruit production and quality, and result in economic losses.
In Jordan the only available information of virus infections in citrus is stemming from a survey conducted in Jordan Valley by Anfoka et al. (2005). They showed that 12.7 and 15.2 % of samples tested by Direct Tissue Blot Immunoassay (TBIA) in the central and northern Jordan Valley respectively were infected with Citrus tristeza virus (CTV). However, during field surveys in Jordan Valley in 2014-2020, different virus symptoms of virus and virus-like diseases were seen on citrus include tree stunting, malformation of branches, trunk and branch bark scaling, trunk and stem pitting of various kinds, gum pockets, gum impregnation and exudation, leaf deformation, leaf chlorosis of several types, vein clearings, ringspots and flecking and fruit abnormalities. Citrus samples were tested by DAS-ELISA for Citrus leaf rugose virus (CLRV), Citrus psorosis virus (CPsV) and Citrus tristeza virus (CTV), or by RT-PCR using primers specific to CLRV, CPsV, Citrus tatter leaf virus (CTLV), CTV, Citrus variegation virus (CVV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd). Results of DAS-ELISA showed the presence of CTV, CPsV and CLRV in the collected samples, and the RT-PCR results confirmed the presence of both CTV and CPsV, beside revealing the presence of HSVd and CEVd for the first time, both being able to induce severe diseases, namely exocortis and cachexia, limiting citrus health, longevity and production (Salem et al., un published data).
Recently the need for a more global and integrated approach for controlling citrus virus and viroid diseases has emerged. High-Throughput Sequencing (HTS) has been broadly used for virus discovery to resolve the disease etiology in many agricultural crops. As HTS facilities and bioinformatics platforms are not available in Jordan and it would be hard to find financial support to carry out or apply for HTS services whether in Jordan or abroad, we are submitting a proposal in the context of the European Virus Archive-Global (EVAg) project, to apply to the EVAg-TNA initiative and perform a virus-discovery analysis of our citrus samples to investigate the presence of (unknown) viruses, viroids and other pathogens possibly involved in the citrus diseases currently observed in citrus growing areas in Jordan. Besides being of high scientific relevance and contributing to increase knowledge on putative citrus disease-causing agents in Jordan, the results may eventually support initiatives to counteract them and preserve citrus production in Jordan.
Citrus is one of the most important fruit crops in Jordan. The most important citrus species grown in Jordan are sweet orange, mandarins and mandarin-like citrus, lemon and grapefruit. Approximately 98% of all these species are planted on sour orange root-stock, and most of the citrus cultivars are grown as grafted plants. As a consequence, virus pathogens transmitted by grafting as well as by insect vectors can cause diseases which finally affect fruit production and quality, and result in economic losses.
In Jordan the only available information of virus infections in citrus is stemming from a survey conducted in Jordan Valley by Anfoka et al. (2005). They showed that 12.7 and 15.2 % of samples tested by Direct Tissue Blot Immunoassay (TBIA) in the central and northern Jordan Valley respectively were infected with Citrus tristeza virus (CTV). However, during field surveys in Jordan Valley in 2014-2020, different virus symptoms of virus and virus-like diseases were seen on citrus include tree stunting, malformation of branches, trunk and branch bark scaling, trunk and stem pitting of various kinds, gum pockets, gum impregnation and exudation, leaf deformation, leaf chlorosis of several types, vein clearings, ringspots and flecking and fruit abnormalities. Citrus samples were tested by DAS-ELISA for Citrus leaf rugose virus (CLRV), Citrus psorosis virus (CPsV) and Citrus tristeza virus (CTV), or by RT-PCR using primers specific to CLRV, CPsV, Citrus tatter leaf virus (CTLV), CTV, Citrus variegation virus (CVV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd). Results of DAS-ELISA showed the presence of CTV, CPsV and CLRV in the collected samples, and the RT-PCR results confirmed the presence of both CTV and CPsV, beside revealing the presence of HSVd and CEVd for the first time, both being able to induce severe diseases, namely exocortis and cachexia, limiting citrus health, longevity and production (Salem et al., un published data).
Recently the need for a more global and integrated approach for controlling citrus virus and viroid diseases has emerged. High-Throughput Sequencing (HTS) has been broadly used for virus discovery to resolve the disease etiology in many agricultural crops. As HTS facilities and bioinformatics platforms are not available in Jordan and it would be hard to find financial support to carry out or apply for HTS services whether in Jordan or abroad, we are submitting a proposal in the context of the European Virus Archive-Global (EVAg) project, to apply to the EVAg-TNA initiative and perform a virus-discovery analysis of our citrus samples to investigate the presence of (unknown) viruses, viroids and other pathogens possibly involved in the citrus diseases currently observed in citrus growing areas in Jordan. Besides being of high scientific relevance and contributing to increase knowledge on putative citrus disease-causing agents in Jordan, the results may eventually support initiatives to counteract them and preserve citrus production in Jordan.
Citrus is one of the most important fruit crops in Jordan. The most important citrus species grown in Jordan are sweet orange, mandarins and mandarin-like citrus, lemon and grapefruit. Approximately 98% of all these species are planted on sour orange root-stock, and most of the citrus cultivars are grown as grafted plants. As a consequence, virus pathogens transmitted by grafting as well as by insect vectors can cause diseases which finally affect fruit production and quality, and result in economic losses.
In Jordan the only available information of virus infections in citrus is stemming from a survey conducted in Jordan Valley by Anfoka et al. (2005). They showed that 12.7 and 15.2 % of samples tested by Direct Tissue Blot Immunoassay (TBIA) in the central and northern Jordan Valley respectively were infected with Citrus tristeza virus (CTV). However, during field surveys in Jordan Valley in 2014-2020, different virus symptoms of virus and virus-like diseases were seen on citrus include tree stunting, malformation of branches, trunk and branch bark scaling, trunk and stem pitting of various kinds, gum pockets, gum impregnation and exudation, leaf deformation, leaf chlorosis of several types, vein clearings, ringspots and flecking and fruit abnormalities. Citrus samples were tested by DAS-ELISA for Citrus leaf rugose virus (CLRV), Citrus psorosis virus (CPsV) and Citrus tristeza virus (CTV), or by RT-PCR using primers specific to CLRV, CPsV, Citrus tatter leaf virus (CTLV), CTV, Citrus variegation virus (CVV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd). Results of DAS-ELISA showed the presence of CTV, CPsV and CLRV in the collected samples, and the RT-PCR results confirmed the presence of both CTV and CPsV, beside revealing the presence of HSVd and CEVd for the first time, both being able to induce severe diseases, namely exocortis and cachexia, limiting citrus health, longevity and production (Salem et al., un published data).
Recently the need for a more global and integrated approach for controlling citrus virus and viroid diseases has emerged. High-Throughput Sequencing (HTS) has been broadly used for virus discovery to resolve the disease etiology in many agricultural crops. As HTS facilities and bioinformatics platforms are not available in Jordan and it would be hard to find financial support to carry out or apply for HTS services whether in Jordan or abroad, we are submitting a proposal in the context of the European Virus Archive-Global (EVAg) project, to apply to the EVAg-TNA initiative and perform a virus-discovery analysis of our citrus samples to investigate the presence of (unknown) viruses, viroids and other pathogens possibly involved in the citrus diseases currently observed in citrus growing areas in Jordan. Besides being of high scientific relevance and contributing to increase knowledge on putative citrus disease-causing agents in Jordan, the results may eventually support initiatives to counteract them and preserve citrus production in Jordan.
Citrus is one of the most important fruit crops in Jordan. The most important citrus species grown in Jordan are sweet orange, mandarins and mandarin-like citrus, lemon and grapefruit. Approximately 98% of all these species are planted on sour orange root-stock, and most of the citrus cultivars are grown as grafted plants. As a consequence, virus pathogens transmitted by grafting as well as by insect vectors can cause diseases which finally affect fruit production and quality, and result in economic losses.
In Jordan the only available information of virus infections in citrus is stemming from a survey conducted in Jordan Valley by Anfoka et al. (2005). They showed that 12.7 and 15.2 % of samples tested by Direct Tissue Blot Immunoassay (TBIA) in the central and northern Jordan Valley respectively were infected with Citrus tristeza virus (CTV). However, during field surveys in Jordan Valley in 2014-2020, different virus symptoms of virus and virus-like diseases were seen on citrus include tree stunting, malformation of branches, trunk and branch bark scaling, trunk and stem pitting of various kinds, gum pockets, gum impregnation and exudation, leaf deformation, leaf chlorosis of several types, vein clearings, ringspots and flecking and fruit abnormalities. Citrus samples were tested by DAS-ELISA for Citrus leaf rugose virus (CLRV), Citrus psorosis virus (CPsV) and Citrus tristeza virus (CTV), or by RT-PCR using primers specific to CLRV, CPsV, Citrus tatter leaf virus (CTLV), CTV, Citrus variegation virus (CVV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd). Results of DAS-ELISA showed the presence of CTV, CPsV and CLRV in the collected samples, and the RT-PCR results confirmed the presence of both CTV and CPsV, beside revealing the presence of HSVd and CEVd for the first time, both being able to induce severe diseases, namely exocortis and cachexia, limiting citrus health, longevity and production (Salem et al., un published data).
Recently the need for a more global and integrated approach for controlling citrus virus and viroid diseases has emerged. High-Throughput Sequencing (HTS) has been broadly used for virus discovery to resolve the disease etiology in many agricultural crops. As HTS facilities and bioinformatics platforms are not available in Jordan and it would be hard to find financial support to carry out or apply for HTS services whether in Jordan or abroad, we are submitting a proposal in the context of the European Virus Archive-Global (EVAg) project, to apply to the EVAg-TNA initiative and perform a virus-discovery analysis of our citrus samples to investigate the presence of (unknown) viruses, viroids and other pathogens possibly involved in the citrus diseases currently observed in citrus growing areas in Jordan. Besides being of high scientific relevance and contributing to increase knowledge on putative citrus disease-causing agents in Jordan, the results may eventually support initiatives to counteract them and preserve citrus production in Jordan.