EVAg

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect castor bean tick nymphs (Ixodes ricinus). The nymphs will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 nymphs will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 nymphs and provided to the end-user.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect adult castor bean ticks (Ixodes ricinus). The ticks will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 ticks will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 ticks and provided to the end-user.

Bundle of freeze-dried tick-borne encephalitis virus (TBEV) isolates representing the diversity of the virus in Central Europe and covering various isolation sources (tick, rodent, milk).

TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis virus[A104] is freeze dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.

TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis virus[A104] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative.

TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis virus[114] is freeze dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.

TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis virus[114] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative.

TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis[Clg223] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative.

TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis[Clg223] is freeze dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.

TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis virus[285] is freeze dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.

TBEV isolate from our collection (IVSAS). This Tick-borne encephalitis virus[285] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative.

This West Nile virus strain is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been fully sequenced.

This West Nile virus strain is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been fully sequenced.

This West Nile virus strain is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been fully sequenced.

Prospect Hill virus prototype strain PH-1. The virus is freeze-dried. Tests for the presence of mycoplasmae were negative. To confirm its identity the partial coding sequence of the virus has been determined.

Prospect Hill virus prototype strain PH-1. The virus is preserved under Viral Storage Medium -80C. Tests for the presence of mycoplasmae were negative.

Dobrava-Belgrade virus strain SK/Aa. This Dobrava-Belgrade virus[Slovakia/Aa] is freeze-dried.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.

This West Nile virus is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been fully sequenced.

Dobrava-Belgrade virus strain Ap1584/Sochi-01. This virus represents Sochi genotype of the Dobrava-Belgrade orthohantavirus species. The virus is preserved under Viral Storage Medium -80C.

Dobrava-Belgrade virus strain Ap1584/Sochi-01. This virus represents Sochi genotype of the Dobrava-Belgrade orthohantavirus species. The virus is freeze-dried. Tests for the presence of mycoplasmae were negative.

West Nile virus isolate.

Chikungunya virus cDNA

Tick-borne encephalitis virus strain Hypr cDNA

Tick-borne encephalitis virus strain Hypr RNA

Sindbis virus original isolate. This Sindbis virus[Sindbis 183] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative.